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	Provedor de dados Animal Sciences (1) BJMBR (1) Autor Alvares,L.E. (1) Carani, Fernanda Regina; Universidade Estadual Paulista (UNESP) (1) Corrente,J.E. (1) Coutinho,L.L. (1) Dal-Pai, Maeli; Universidade Estadual Paulista (UNESP) (1) Duran, Bruno Oliveira da Silva; Universidade Estadual Paulista (UNESP) (1) Mantoani,A. (1) Paula, Tassiana Gutierrez de; Universidade Estadual Paulista (UNESP) (1) Piedade, Warlen Pereira; Universidade Estadual Paulista (UNESP) (1) Mais... Palavra-chave Myogenic regulatory factors (2) 5.06.03.00-0 muscle fibre morphology (1) Arapaima gigas (1) Gene expression (1) Myostatin (1) Quantitative RT-PCR (1) Real time PCR (1) Skeletal muscle growth (1) Standard-curve (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2013 (1) 2003 (1) País Brazil (2) Idioma Inglês (1) Português (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Morphology and expression of genes related to skeletal muscle growth in juveniles of pirarucu (Arapaima gigas, Arapaimatidae, Teleostei) - doi: 10.4025/actascianimsci.v35i3.18219 Animal Sciences Carani, Fernanda Regina; Universidade Estadual Paulista (UNESP); Duran, Bruno Oliveira da Silva; Universidade Estadual Paulista (UNESP); Paula, Tassiana Gutierrez de; Universidade Estadual Paulista (UNESP); Piedade, Warlen Pereira; Universidade Estadual Paulista (UNESP); Dal-Pai, Maeli; Universidade Estadual Paulista (UNESP). Skeletal muscle growth in the pirarucu (Arapaima gigas) is highly interesting to fish farmers because it provides information about how the mechanism in muscle mass increase, characteristic of the species, is regulated. Pirarucu has specific muscle growth that highlights the species’s significance and commercial value. Current research evaluates the morphology and the growth-related gene expression in the red and white skeletal muscles of the pirarucu. Muscle samples were collected from the lateral anterior region and frozen in liquid nitrogen. Histological sections were performed and stained by HE for morphological analysis. Red and white muscle samples were used to determine MyoD, myogenin, and myostatin genes expression by Real-time Polymerase Chain... Tipo: Info:eu-repo/semantics/article Palavras-chave: 5.06.03.00-0 muscle fibre morphology; Myogenic regulatory factors; Myostatin; Skeletal muscle growth; Arapaima gigas; Real time PCR. Ano: 2013 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/18219 Standard-curve competitive RT-PCR quantification of myogenic regulatory factors in chicken embryos BJMBR Alvares,L.E.; Mantoani,A.; Corrente,J.E.; Coutinho,L.L.. The reverse transcription-polymerase chain reaction (RT-PCR) is the most sensitive method used to evaluate gene expression. Although many advances have been made since quantitative RT-PCR was first described, few reports deal with the mathematical bases of this technique. The aim of the present study was to develop and standardize a competitive PCR method using standard-curves to quantify transcripts of the myogenic regulatory factors MyoD, Myf-5, Myogenin and MRF4 in chicken embryos. Competitor cDNA molecules were constructed for each gene under study using deletion primers, which were designed to maintain the anchorage sites for the primers used to amplify target cDNAs. Standard-curves were prepared by co-amplification of different amounts of target cDNA... Tipo: Info:eu-repo/semantics/article Palavras-chave: Quantitative RT-PCR; Standard-curve; Myogenic regulatory factors; Gene expression. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001200004 Registros recuperados: 2 Primeira ... 1 ... Última

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